Uhlalutyo lomkhondo lweesampulu zolwelo lunoluhlu olubanzi lwezicelo kwisayensi yobomi kunye nokubeka iliso kokusingqongileyo.Kulo msebenzi, siye saqulunqa i-compact kunye ne-photometer engabizi kakhulu esekelwe kwi-metal waveguide capillaries (MCCs) ukwenzela ukuzimisela kwe-ultrasensitive of absorption.Indlela ye-optical inokwandiswa kakhulu, kwaye ide kakhulu kunobude bomzimba be-MWC, kuba ukukhanya okusasazwe ngamacangci agudileyo emacaleni esinyithi kunokuqulathwa ngaphakathi kwe-capillary kungakhathaliseki ukuba i-angle yesiganeko.Ugxininiso oluphantsi njenge-5.12 nM lunokufezekiswa kusetyenziswa ii-reagents eziqhelekileyo ze-chromogenic ngenxa ye-amplification entsha engekho-linear kunye nokutshintshwa kwesampuli ngokukhawuleza kunye nokufunyanwa kwe-glucose.
I-Photometry isetyenziselwa ngokubanzi uhlalutyo lomkhondo lweesampulu zolwelo ngenxa yobuninzi bee-reagents ze-chromogenic ezikhoyo kunye nezixhobo ze-semiconductor optoelectronic1,2,3,4,5.Xa kuthelekiswa ne-cuvette-based absorbance determination determination, liquid waveguide (LWC) capillaries reflect (TIR) ngokugcina ukukhanya kweprobe ngaphakathi kwe-capillary1,2,3,4,5.Nangona kunjalo, ngaphandle kokuphucula ngakumbi, umzila we-optical usondele kuphela kubude bomzimba we-LWC3.6, kwaye ukwandisa ubude be-LWC ngaphaya kwe-1.0 m kuya kubandezeleka ngenxa yokunciphisa ukukhanya okunamandla kunye nomngcipheko omkhulu weebhubhu, njl.3, 7. Ngokuphathelele kwiseli ecetywayo ye-multi-reflection yokuphucula indlela ye-optical, umda wokufumanisa uphuculwe kuphela yinto ye-2.5-8.9.
Kukho okwangoku iindidi ezimbini eziphambili ze-LWC, ezizezi, i-Teflon AF capillaries (ene-refractive index ye-~1.3 kuphela, engaphantsi kuneyamanzi) kunye ne-silica capillaries eqatywe ngeTeflon AF okanye iifilimu zetsimbi1,3,4.Ukufezekisa i-TIR kwi-interface phakathi kwezinto ze-dielectric, izixhobo ezine-low refractive index kunye ne-high incience angle angles zifunekayo3,6,10.Ngokubhekiselele kwi-Teflon AF capillaries, iTeflon AF iyaphefumla ngenxa yesakhiwo sayo se-porous3,11 kwaye inokufunxa ixabiso elincinci lezinto kwiisampuli zamanzi.Kwiicapillaries zequartz ezigqunywe ngaphandle ngeTeflon AF okanye isinyithi, isalathisi se-quartz (1.45) siphezulu kuneesampuli ezininzi zolwelo (umz. 1.33 yamanzi)3,6,12,13.Kwii-capillaries ezifakwe kwifilimu yensimbi ngaphakathi, iipropati zokuthutha ziye zafundwa14,15,16,17,18, kodwa inkqubo yokugqoka iyinkimbinkimbi, ubuso befilimu yensimbi inesakhiwo esinqabileyo kunye ne-porous4,19.
Ukongeza, ii-LWCs zorhwebo (i-AF Teflon Coated Capillaries kunye ne-AF Teflon Coated Silica Capillaries, iWorld Precision Instruments, Inc.) zinezinye izinto ezingeloncedo, ezinje: kwiimpazamo..Umthamo omkhulu ofileyo we-TIR3, 10, (2) i-T-connector (ukudibanisa i-capillaries, iifibers, kunye ne-inlet / outlet tubes) inokubamba ama-bubbles emoyeni10.
Kwangaxeshanye, ukumiselwa kwamanqanaba eglucose kubaluleke kakhulu ekuxilongeni isifo seswekile, i-cirrhosis yesibindi kunye nesifo sengqondo20.kunye neendlela ezininzi zokubona ezifana nephotometry (kubandakanya i-spectrophotometry 21, 22, 23, 24, 25 kunye ne-colorimetry kwiphepha 26, 27, 28), igalvanometry 29, 30, 31, fluorometry 32, 33, 34, 35, optical 6 polari3 umphezulu plasmon resonance.I-37, i-Fabry-Perot cavity 38, i-electrochemistry 39 kunye ne-capillary electrophoresis 40,41 njalo njalo.Nangona kunjalo, uninzi lwezi ndlela zifuna izixhobo ezibiza kakhulu, kwaye ukufumanisa i-glucose kwiindawo ezininzi ze-nanomolar zihlala zingumngeni (umzekelo, kwimilinganiselo ye-photometric21, 22, 23, 24, 25, 26, 27, 28, i-concentration ephantsi ye-glucose).umda wawuyi-30 nM kuphela xa iPrussian blue nanoparticles yayisetyenziswa njenge-peroxidase mimics).Uhlalutyo lwe-Nanomolar glucose luhlala lufuneka kwizifundo zeselula ezikwinqanaba le-molekyuli ezifana nokuthintela ukukhula komhlaza wesibeleko somntu42 kunye nokuziphatha kwe-CO2 yokulungiswa kweProchlorococcus elwandle.
Ixesha lokuposa: Nov-26-2022